Giuseppe Inesi, MD

Education  |  Academic Appointments  |  Research Activity and Interests  |  Figures  |  Publications

Education

1954 University of Modena, Italy Doctor in Medicine
1960 University of Bologna, Italy Docence (Ph.D)
1958-62 University of Pennsylvania, Post doctoral Fellow
1963-65 University of California San Francisco, Postdoctoral Fellow

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Academic Appointments

1965-70 Established Investigator of the American Heart Association

1965-69 University of California Assistant Professor
San Francisco, California Cardiovascular Research Institute

1969-72 Carnegie-Mellon University Tenured Associate Professor
Pittsburgh, Pennsylvania (Biochemistry)

1972-81 University of the Pacific Tenured Professor
San Francisco, California (Physiology)

1981-2006 University of Maryland Tenured Professor
Biochemistry and Mol Biol
Chairman (1983-2006)
Baltimore, Maryland

1989-90 Stanford University Visiting Professor
Stanford, California

1996-97 University of California Visiting Professor
San Francisco, California

2005 University of Florence, Italy
Visiting Professor

2006 Calif. Pacific Med. Center Res. Inst.
Senior Scientist

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Research Activity and Interests

1) Mechanisms of free energy transduction in enzymes

2) Active transport (Ca²⁺) in biomembranes

3) Excitation-contraction coupling in skeletal and cardiac muscle

4) Ca²⁺ and signal transduction

5) Gene transfer in Eukaryotic cells

Dr Inesi's laboratory has been engaged in a long term research project on Molecular and Cellular Aspects of the Ca²⁺ Transport ATPase. This enzyme is a membrane bound protein that utilizes ATP for active transport of Ca²⁺, and is required for storage of Ca²⁺ in intracellular stores. In turn, release of the stored Ca²⁺ is required for intracellular signaling and initiation of most cellular functions as a second messenger. Dr Inesi's molecular studies include determination of enzyme and transport kinetics, protein chemistry and derivatization of various amino acid residues, and production of recombinant protein to obtain specific mutations and assess their effects on catalytic and transport function. Cellular studies involve overexpression or silencing of the ATPase gene in cultured cells, and determination of functional consequences with regard to Ca²⁺ signaling and regulation of Ca²⁺ dependent functions. This research is currently supported by a five year National Institutes of Health (NIHLBI) award. Recent articles from Dr Inesi's laboratory include:

9. Inesi G., Sumbilla, C., and Kirtley, M.E. Relationships of molecular structure and function in the Ca ²⁺ transport. ATPase. Physiol. Rev. 7(3):749-760 (1990).

23. Toyoshima, C. and Inesi G., Structural Basis of Ion Pumping by Ca²⁺ ATPase of Sarcoplasmic Reticulum. In: “Annual Review of Biochemistry” 73:269-292 (2004).

172. Ma H, Sumbilla CM, Farrance IK, Klein MG, Inesi G. Cell-specific expression of SERCA, the exogenous Ca2+ transport ATPase, in cardiac myocytes. Am J Physiol Cell Physiol. 2004;286(3):C556-64 (2004)

173. Inesi G, Ma H, Lewis D, Xu C.: Ca ²⁺ occlusion and gating function of Glu309 in the ADP-fluoroaluminate analog of the Ca ²⁺-ATPase phosphoenzyme intermediate. J Biol Chem. 279:31629-37 (2004)

174. Hua S, Xu C, Ma H, Inesi G: Interference with phosphoenzyme isomerization and inhibition of the Ca²⁺ ATPase (SERCA) by 1,3-dibromo-2,4,6-tri(methylisothiouronium) benzene. J Biol Chem. 280: 17579-17583 (2005)

175. Ma H, Lewis D, Xu C, Inesi G, Toyoshima C. Functional and structural roles of critical amino acids within the “N”, “P” and “A” domains of the Ca²⁺ ATPase headpiece. BIOCHEMISTRY, 44:8090-8100 (2005)

176. Obara K., Miyiashita N., Xu C., Toyoshima I, Sugita Y. Inesi G., Toyoshima C.: Structural role of countertransport revealed in Ca²⁺ pump crystals structure in the absence of Ca²⁺. Proc. Nat. Acad. Sci. 102: 14489-14496 (2005)

177. Inesi G, Lewis D., Ma H., Prasad A. and Toyoshima C.
Concerted conformational effects of Ca 2+ and ATP are required for activation of sequential reactions in the Ca ²⁺ ATPase (SERCA) catalytic cycle. BIOCHEMISTRY 2006, November issue.

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Figures

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Publications

Click here for List of publications by Giuseppe Inesi, MD. in PubMed.

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